Protease Inhibitor Cocktail (EDTA free)
TOOLS protease inhibitor cocktail inhibits the degradation of proteins in tissue or cell extracts by endogenous proteases. It is useful for exploring particular processes that involve blocking the activity of specific proteases. This product can be used in mammalian cell lysates or tissue extracts to increase protein stability. As the name indicates, the cocktail functions to inhibit proteases that would degrade either phosphorylated or non-phosphorylated protein substrates. TOOLS Protease Inhibitor Cocktail is a mixture of protease inhibitors for protection of protein integrity.
Features
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Easy to use-100X solution
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EDTA free-formulated without EDTA, a metalloproteinase inhibitor
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Economical-more cost-effective
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TOOLS Protease inhibitor is blend of protease inhibitors for protection of protein integrity.
Component
|
Target
|
Inhibitor Type
|
AEBSF
|
Serine proteases
|
Irreversible
|
Aprotinin
|
Serine proteases
|
Reversible
|
Bestatin
|
Aminopeptidases
|
Reversible
|
E-64
|
Cysteine proteases
|
Irreversible
|
Leupeptin
|
Serine and cysteine proteases
|
Reversible
|
Pepstatin A
|
Aspartic acid-proteases
|
Reversible
|
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YANG, Wen-Bin, et al. Dehydroepiandrosterone Induces Temozolomide Resistance Through Modulating Phosphorylation and Acetylation of Sp1 in Glioblastoma. Molecular neurobiology, 2018, 1-13.
Q1. Protease inhibitor cocktail cannot dissolve in DMSO
A: We suggest you use shaker and dissovle in 4 degrees. Short sonication can also help.
Q2. Can Protease Inhibitor Cocktail dissolve in 100ml extract buffer?
A: No. We suggest you dissolve in 10ml extract buffer according to protocol.
A: We suggest you use shaker and dissovle in 4 degrees. Short sonication can also help.
Q2. Can Protease Inhibitor Cocktail dissolve in 100ml extract buffer?
A: No. We suggest you dissolve in 10ml extract buffer according to protocol.